polyclonal anti human growth hormone antibody  (Proteintech)

Journal: Endocrinology

Article Title: POU1F1/Pou1f1 c.143-83A > G Variant Disrupts the Branch Site in Pre-mRNA and Leads to Dwarfism

doi: 10.1210/endocr/bqac198

Figure Lengend Snippet: (A) Stereomicroscope images of pituitary glands of Pou1f1 WT/WT and Pou1f1 GE/GE mice at 12 weeks of age. Scale bar, 1 mm. (B) Pituitary gland weight per body weight of Pou1f1 WT/WT , Pou1f1 WT/GE , and Pou1f1 GE/GE mice. Data represent the mean ± SD. * P < 0.05 vs Pou1f1 WT/WT mice. (C) Histology of pituitary glands at 12 weeks of age. Upper images show hematoxylin and eosin staining. Lower images depict immunostaining with anti-GH antibody. Scale bar, 100 μm. (D, E) Serum IGF-1 and thyroxine values of 12 weeks of age, respectively. Data represent the mean ± SD. * P < .05 vs Pou1f1 WT/WT mice. ** P < .01; n.s., not significant.

Article Snippet: Pituitary sections were incubated with 2% skim milk in phosphate-buffered saline for 1 hour at room temperature, and incubated with the polyclonal goat anti-GH antibody (20 ng/μL; catalog no. AF1067; RRID:AB_354573; R&D systems, MN, USA) overnight at 4 °C in a humidified chamber.

Techniques: Staining, Immunostaining